Cell Studies with Fluorescence Correlation Spectroscopy :: MaterialsViews
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Cell Studies with Fluorescence Correlation Spectroscopy
by Guido Fuchs published: 2011-01-07 thumbnail image: Cell Studies with Fluorescence Correlation Spectroscopy
Text contributed by K. Maedefessel-Herrmann.
Since its advent more than 30 years ago, Fluorescence Correlation Spectroscopy (FCS) has developed into a versatile single molecule technique with numerous novel extensions, suiting a broad spectrum of applications. The high temporal and spatial resolution in combination with noninvasiveness and specificity makes it an ideal tool to study the dynamics, local concentrations and interactions of labeled molecules in vivo. Petra Schwille and colleagues from BIOTEC, Dresden University of Technology, and Max Planck Institute for Biophysical Chemistry, Göttingen (Germany) now give a comprehensive review how FCS and Fluorescence Cross-correlation Spectroscopy (FCCS) can be applied to the living cell and give an overview on a range of recent technical advancements. Practical issues an
